Lenti-APEX2-EGFP-SFV nsP3

LOCUS       Lenti-APEX2-EGFP-SFV nsP3       11190 bp DNA     circular SYN 24-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..11190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2210..2512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2513..2716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2755..2764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2761..3477
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3471..3495)
                     /label=p61-312
     primer_bind     3481..3519
                     /label=p61-308
     regulatory      3496..3505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3505..3528
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3529..4275
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     primer_bind     4165..4184
                     /label=Seq-AP-F
     CDS             4282..4308
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(4284..4308)
                     /label=P61-314
     primer_bind     complement(4285..4323)
                     /label=p61-310
     primer_bind     4294..4332
                     /label=p61-306
     CDS             4309..5754
                     /codon_start=1
                     /label=SFV nsP3 (human codon)
                     /translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
                     KGAATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
                     AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDMRTAVE
                     LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
                     LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERIARLRSHQV
                     KSMVVCSSFPLPKYHVDGVQKVKCEKGLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
                     LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
                     ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
                     VDALASGITFGDFDDVLRLGRAGA"
     primer_bind     4832..4855
                     /label=Seq-C3-1207-F
     primer_bind     complement(5734..5772)
                     /label=p61-307
     primer_bind     5758..5782
                     /label=p61-311
     promoter        5768..6267
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5803..5826)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6200..6219
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6294..7088
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6348..6367)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6958..6977
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7112..7700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7165..7185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7583..7594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7908..8541
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8670..8686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8670..8686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8683..8705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8694..8710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8718..8748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8763..8784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8901..8918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9072..9657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9152..9171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9828..10688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10451..10470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10689..10793)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10841..10975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10878..10897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10932..10951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggg ttacataact
     2221 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2281 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2341 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2401 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2461 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2521 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2581 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2641 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2701 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
     2761 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2821 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2881 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2941 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3001 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3061 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3121 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3181 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3241 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3301 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3361 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3421 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtac
     3481 tcagatctcg agctcgccac catggactac aaggatgacg acgataaggg aaagtcttac
     3541 ccaactgtga gtgctgatta ccaggacgcc gttgagaagg cgaagaagaa gctcagaggc
     3601 ttcatcgctg agaagagatg cgctcctcta atgctccgtt tggcattcca ctctgctgga
     3661 acctttgaca agggcacgaa gaccggtgga cccttcggaa ccatcaagca ccctgccgaa
     3721 ctggctcaca gcgctaacaa cggtcttgac atcgctgtta ggcttttgga gccactcaag
     3781 gcggagttcc ctattttgag ctacgccgat ttctaccagt tggctggcgt tgttgccgtt
     3841 gaggtcacgg gtggacctaa ggttccattc caccctggaa gagaggacaa gcctgagcca
     3901 ccaccagagg gtcgcttgcc cgatcccact aagggttctg accatttgag agatgtgttt
     3961 ggcaaagcta tggggcttac tgaccaagat atcgttgctc tatctggggg tcacactatt
     4021 ggagctgcac acaaggagcg ttctggattt gagggtccct ggacctctaa tcctcttatt
     4081 ttcgacaact catacttcac ggagttgttg agtggtgaga aggaaggtct ccttcagcta
     4141 ccttctgaca aggctctttt gtctgaccct gtattccgcc ctctcgttga caaatatgca
     4201 gcggacgaag atgccttctt tgctgattac gctgaggctc accaaaagct ttccgagctt
     4261 gggtttgctg atgccctgca gctgcctccc ctggagcgcc tgaccctggc tcctagttat
     4321 cgggtgaagc gcgcggacat tgctacttgt accgaagccg ccgtagtcaa cgccgccaat
     4381 gctcgcggca ccgtgggcga tggagtctgc cgggccgtcg cgaaaaagtg gccgagtgcg
     4441 ttcaaaggcg cggcaacccc agttgggaca attaagaccg tcatgtgtgg cagctatccg
     4501 gtcatccacg ccgtcgcccc taacttctca gcaacaaccg aggctgaagg ggaccgggaa
     4561 ctcgccgcgg tatatagagc ggtggcagcc gaggtaaaca gattgtcctt gagttccgtt
     4621 gcaattcccc ttcttagcac aggtgtgttt agcggtgggc gcgacagact gcagcagtcc
     4681 ctgaaccatc tctttactgc catggatgcg acggatgcgg atgtcaccat ctattgccgc
     4741 gataagagtt gggagaagaa gattcaagag gccattgata tgaggaccgc agtagagctg
     4801 ctgaatgacg acgtagagct tacgacagac ctggtcagag ttcatcccga cagttccctc
     4861 gttggaagaa agggttacag cacgactgac ggatccttgt actcatactt tgaaggtact
     4921 aagttcaacc aagctgcgat tgatatggcc gaaattttga cgttgtggcc acgcctccaa
     4981 gaggcgaacg agcaaatatg tctgtatgct ctcggggaga ctatggacaa cattcgaagt
     5041 aaatgcccag tcaacgattc agattctagc actcccccta ggactgtccc ctgcttgtgt
     5101 aggtacgcca tgacggccga gcgaatagct cggcttcgct ctcatcaagt caaatcaatg
     5161 gtggtatgtt caagtttccc ccttccgaaa taccatgtag acggggtcca gaaagtcaag
     5221 tgcgaaaaag gtcttttgtt cgatcctact gtaccctctg tagtctctcc gaggaagtat
     5281 gccgctagta ccacagatca ctccgacagg agtttgcggg ggttcgacct tgattggacc
     5341 actgactctt cctcaacggc tagtgatacg atgtcattgc caagcctgca atcatgtgac
     5401 attgacagta tctatgagcc gatggctccc attgtggtca cggctgatgt acatccagaa
     5461 cccgctggaa tcgcagacct tgcggctgac gttcaccccg aaccagcaga tcacgttgac
     5521 ctggagaatc cgattcctcc tccgaggcct aagagagccg cgtacctggc atcacgggcg
     5581 gctgaacggc ccgtccctgc gccaaggaaa cccactcccg ccccacgcac tgcatttaga
     5641 aataagctcc ccctgacttt cggagatttc gatgaacatg aagtagacgc gctcgctagt
     5701 gggataactt ttggagattt tgatgacgtc ctccgactgg gaagagctgg ggcttgagaa
     5761 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     5821 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     5881 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     5941 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     6001 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     6061 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     6121 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     6181 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     6241 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttcctagg accatgattg
     6301 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6361 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6421 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     6481 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6541 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6601 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6661 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6721 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6781 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     6841 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6901 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6961 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7021 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7081 tcttctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7141 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7201 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7261 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7321 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7381 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7441 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7501 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7561 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7621 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7681 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     7741 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7801 gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta
     7861 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     7921 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7981 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     8041 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     8101 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     8161 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     8221 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     8281 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     8341 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8401 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8461 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8521 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8581 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     8641 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8881 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9481 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9601 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10141 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10801 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    10861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10981 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11041 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11101 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
    11161 ttgttaaata tgtactacaa acttagtagt
//